目的 建立一测多评(QAMS)的泽泻多指标质量控制方法,并验证该方法在泽泻含量测定中应用的可行性和技术适应性。方法 采用HPLC,以23-乙酰泽泻醇B为内参物,计算泽泻醇A、24-乙酰泽泻醇A和泽泻醇B的相对校正因子,并利用该校正因子计算含量,同时与采用外标法测定4个三萜类成分的含量进行比较。结果 建立的校正因子重现性良好,31批泽泻药材中4个三萜类的含量计算值与外标法实测值间(RSD ≤ 3.52%)无显著差异。结论 本实验所建立的一测多评法稳定、准确,可用于泽泻三萜类成分的质量控制。
Abstract
OBJECTIVE To develop a simultaneous quantitative analysis method of four triterpenoids in Alismatis Rhizoma by multi-components by single maker (QAMS). METHODS Four main triterpenoids (alisol A, alisol A 24-acetate, alisol B and alisol B 23-acetate) were selected as analytes to evaluate the quality of Alismatis Rhizoma. Alisol B 23-acetate was used as the internal reference standard and three relative correction factors (RCFs) to alisol B 23-acetate were calculated. These RCFs were obtained by high performance liquid chromatography coupled with diode array detector under various chromatographic conditions. Then 31 batches of Alismatis Rhizoma samples collected from different areas were determined by both external standard method (ESM) and QAMS. RESULTS The RCFs were obtained with good reproducibility (RSD ≤ 2.25%). No obvious differences were observed between the analysis results of QAMS method and ESM method (RSD ≤ 3.52%). CONCLUSION The established QAMS method is reliable and feasible for simultaneous analysis of four triterpenoids and could be used for quality control of Alismatis Rhizoma.
关键词
泽泻 /
三萜 /
一测多评 /
质量控制
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Key words
Alismatis Rhizoma /
triterpenoids /
QAMS /
quality control
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中图分类号:
R917
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参考文献
[1] Ch. P (2010) Vol Ⅰ(中国药典 2010 年版.一部). 2010: 212-213.
[2] QIU J F,LIN J,XU W, et al. HPLC-DAD-ELSD Simultaneous determination of four triterpenoids in Rhizoma alismatis. Medicinal Plant, 2014,5(5): 22-26.
[3] XU J J, XU C, LIU B, et al. Simultaneous determination of four flavonoid glycosides in mentha haplocaly x Briq. by a quantitative analysis of multi-components by single marker . Chin Pharm J (中国药学杂志), 2014, 49(3): 234-239.
[4] HE F C, LI S X, ZHAO Z Q, et al. Simultaneous quantitative analysis of four lignanoids in Schisandra chinensis by quantitative analysis of multi- components by single marker . Acta Pharm Sin(药学学报), 2014, 47(20): 930-933.
[5] FENG W H, YANG F, WANG Z M, et al. Simultaneous determination of flavones in Shuanghuanglian Preparations by both QAMS and external standard method. Chin Pharm J (中国药学杂志), 2012, 49(3): 1665-1669.
[6] CHEN J, WANG S M, MENG J, et al. Simultaneous quantitative analysis of five alkaloids in Sophora flavescens by multi-components assay by single marker . China J Chin Mater Med (中国中药杂志), 2013, 38(9): 1406-1410.
[7] WANG Z M, QIAN Z Z, ZHANG Q W, et al. The guidelines for establishment of QAMS . China J Chin Mater Med(中国中药杂志), 2011, 36(6): 657-658.
[8] WANG Z M, GAO M H, FU X T, et al. Multi-components quantitation by one marker new method for quality evaluation of Chinese herbal medicine . China J Chin Mater Med(中国中药杂志), 2006, 31(23): 1925-1928.
[9] YOSHIJIRO N, YOHKO S, MASUMI K, et al. Terpenoids of Alisma orientale rhizome and the crude drug alismatis rhizoma . Phytochemistry, 1994, 36(1): 119-127.
MAKABEL B, ZHAO Y, WANG B, et al. Stability and structure studies on alisol a 24-acetate. Chem Pharm Bull, 2008, 56(1): 41-45.
ZHENG Y F, ZHU Y L, PENG G P, et al. Transformation of alisol B 23-acetate in processing of Alisma orientalis.Chin Tradit Herb Drugs(中草药), 2006, 37(10):1479-1482.
YOSHIKAWA M, YAMAGUCHI S, CHATANI N, et al. Crud drugs from aquatic plants. Ⅲ. Quantitative analysis of triterpene constituents in Alismatis Rhizoma by means of high performance liquid chromatography on the chemical change of the constituents during Alismatis Rhizoma processing . Chem Pharm Bull, 1994, 114(4): 241-247.
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脚注
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基金
国家自然科学基金资助项目(U1205022);国家“十二五”科技支撑计划(2011BAI01B06);福建省自然基金资助项目(2014J01352);福建省卫生厅青年科研资助课题(2013-2-55);福建中医药大学校管课题(X2014107-学科X2015011-平台);福建省大学生创新训练项目(201410393034)
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